Wind energy is rapidly growing as a renewable source of energy but is not neutral for wildlife, especially bats.Whereas most studies have focused on bat mortality through collision, very few have quantiﬁed the loss of habitat use resulting from the potential negative impact of wind turbines, and none of them for hub heights higher than 55 m. Such impacts could durably aﬀect populations, creating a need for improvement of knowledge to integrate this concern in implementation strategies. We quantiﬁed the impact of wind turbines at diﬀerent distances on the activity of 11 bat taxa and 2 guilds. We compared bat activity at hedgerows (207 sites) located at a distance of 0–1000 m from wind turbines (n = 151) of 29 wind farms in an agricultural region in the autumn (overall 193,980 bat passes) using GLMMs. We found a signiﬁcant negative eﬀect of proximity to turbines on activity for 3 species (Barbastella barbastellus, Nyctalus leisleiri, Pipistrellus pipistrellus), 2 species-groups (Myotis spp., Plecotus spp.) and 2 guilds (fast-ﬂying and gleaner). Bat activity within 1000 m of wind turbines by gleaners and fast-ﬂying bats is reduced by 53.8% and 19.6%, respectively. Our study highlighted that European re-commendations (at least 200 m from any wooded edge) to limit mortality events likely strongly underestimate the loss of bat activity. The current situation is particularly worrying, with 89% of 909 turbines established in a region that does not comply with recommendations, which themselves are far from suﬃcient to limit the loss of habitat use.