We developed a simple and reliable genetic method to determine sex in bats from the Vespertilionidae and Molossidae families. Polymerase chain reaction was used to amplify a portion of the introns within the zinc-finger-X (Zfx) and zinc-finger-Y (Zfy) genes. We designed primers to produce size variation between the Zfx and Zfy products that could be visualized using gel electrophoresis. Using an example from our wind-wildlife research, we show how sex data generated using this method are superior to sex data based on external morphology. Our method allows for the generation of sex data across a wide range of bats that can be used to address key questions in wildlife forensics, behavioural ecology, conservation and evolutionary biology.
Advances in Sex Determination in Bats and its Utility in Wind-Wildlife Studies
Title: Advances in Sex Determination in Bats and its Utility in Wind-Wildlife Studies
May 06, 2013
Journal: Molecular Ecology Resources
Korstian, J.; Hale, A.; Bennett, V.; Williams, D. (2013). Advances in Sex Determination in Bats and its Utility in Wind-Wildlife Studies. Molecular Ecology Resources, 13, 776-780.